Willow bark extract (BNO1455) and its fractions suppress growth and induce apoptosis in human colon and lung cancer cells
Katarina Hostanska PhDa, Guido Jürgenliemk PhDb, Gudrun Abel PhDc, Adolf Nahrstedt PhDb and Reinhard Saller MDa
aUniversity Hospital Zürich, Department of Internal Medicine, Institute for Complementary Medicine, FGel 102, Rämistrasse 100, CH-8091 Zürich, Switzerland
bUniversity of Muenster, Institute of Pharmaceutical Biology and Phytochemistry, Hittorfstrasse 55, D-48149 Muenster, Germany
cBionorica AG, Phytoneering Company, Department of Research and Development, Kerschensteinerstrasse 11-15, D-92318 Neumarkt, Germany
Background: Recently, there have been extensive efforts to evaluate the chemopreventive role of substances present in natural products. The aim of this study was to examine the effects of the main groups of compounds (salicylalcohol derivates, flavonoids, proanthocyanidins), and salicin isolated from willow bark extract BNO 1455 on proliferation and apoptosis in human colon and cancer cells.
Methods: We used human colon cyclooxygenase-2 (COX-2)-positive HT 29 and (COX-2)-negative HCT 116 or lung COX-2 proficient A 549 and low COX-2 expressing SW2 cells. After treatment for 72 h with various concentrations of single substances and acetylsalicylic acid (ASA) as control, inhibition of cell growth and cytotoxicity were measured by colorimetric WST-1 assay and propidium iodide uptake by flow cytometry, respectively.
Apoptotic cells were identified by annexin V adhesion using flow cytometry. Results: Studies on dose-dependent effects of BNO 1455 and its fractions showed anti-proliferative activity of all compounds with 50% maximal growth inhibitory concentrations (GI50) between 33.3 and 103.3 μg/ml for flavonoids and proanthocyanidins fractions and 50.0–243.0 μg/ml for salicylalcohol derivates and extract.
Apoptosis induction was confirmed by annexin V adherence and analysis of cell morphology based on light scattering characteristics using flow cytometry in all cell lines at GI50. Conclusions: We showed that willow bark extract BNO 1455 an its fractions inhibit the cell growth and promote apoptosis in human colon and lung cancer cell lines irrespective of their COX-selectivity.
Cancer Detection and Prevention, proof, 4/07
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