Uncaria tomentosa extracts dffrntlly rglts exprssn  IL-1alpha & TNF-a

Treatment of THP-1 cells with Uncaria tomentosa extracts differentially regulates the expression of IL-1alpha and TNF-alpha

Lisa Allen-Halla, b, Pablo Canoa, John T. Arnasonc, Rosario Rojasd, Olga Locke and Robert M. Lafreniea, b, f, ,

aRegional Cancer Program, Sudbury Regional Hospital, 41 Ramsey Lake Road, Sudbury Ont., Canada P3E 5J1 bProgram in Biomolecular Sciences, Laurentian University, 935 Ramsey Lake Road, Sudbury, Ont., Canada P3E 2C6 cDepartment of Biology, University of Ottawa, 150 Louis Pasteur Priv., Ottawa, Ont., Canada K1N 6N5 dFacultad de Ciencias y Filosof237;a, Universidad Peruana Cayetano Heredia, Lima, Per250; ePontificia Universidad Catolica Del Peru, Avenida Universitaria, Cuadra 18, San Miguel, Lima, Peru fNorthern Ontario School of Medicine, 935 Ramsey Lake Road, Sudbury, Ont., Canada P3E 2C6

Received 16 February 2006; revised 14 July 2006; accepted 31 July 2006. Available online 3 August 2006.

Abstract Uncaria tomentosa, commonly known as cat's claw, is a medicinal plant native to Peru, which has been used for decades in the treatment of various inflammatory disorders. Uncaria tomentosa can be used as an antioxidant, has anti-apoptotic properties, and can enhance DNA repair, however it is best know for its anti-inflammatory properties.

Treatment with Uncaria tomentosa extracts inhibits the production of the pro-inflammatory cytokine, TNF-alpha, which is a critical mediator of the immune response. In this paper, we showed that treatment of THP-1 monocyte-like cells with Uncaria tomentosa extracts inhibited the MAP kinase signaling pathway and altered cytokine expression.

Using ELISA assays, we showed that treatment with Uncaria tomentosa extracts augmented LPS-dependent expression of IL-1alpha by 2.4-fold, while inhibiting the LPS-dependent expression of TNF-alpha by 5.5-fold. We also showed that treatment of LPS-stimulated THP-1 cells with Uncaria tomentosa extracts blocked ERK1/2 and MEK1/2 phosphorylation in a dose-dependent manner.

These data demonstrate that treatment of THP-1 cells with Uncaria tomentosa extracts has opposite effects on IL-1alpha and TNF-alpha secretion, and that these changes may involve effects on the MAP kinase pathway.

Journal of Ethnopharmacology Volume 109, Issue 2 , 19 January 2007, Pages 312-317

doi:10.1016/j.jep.2006.07.039

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