Protective Effect: Andrographis paniculata & Urothelial Toxicity

Protective Effect of Andrographis paniculata and Andrographolide on Cyclophosphamide-Induced Urothelial Toxicity

K. Sheeja, MSc

Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India,

Girija Kuttan, PhD

Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India.

The protective effect of Andrograhis paniculata and andrographolide (ANDLE) against cyclophosphamide (CTX)-induced urothelial toxicity was investigated in this study. Pretreatment of Swiss albino mice with A paniculata extract (10 mg/dose/animal intraperitoneally [ip]) and ANDLE (500 g/dose/animal ip) could significantly reduce CTX (1.5 nmol/kg body weight)-induced urothelial toxicity.

Morphological and histopathological analysis of urinary bladder of CTX-treated mice showed severe inflammation and dark coloration, whereas A paniculata and ANDLE-treated mice showed almost normal bladder morphology.

Elevation of urinary protein level (7.330.3 g/L) by CTX administration was reduced by A paniculata (3.78 0.4 g/L) and ANDLE treatment (4.19 0.1 g/L). Urinary urea N2level, which was elevated after 48 hours of CTX administration (24.25 0.2 g/L) was found to be reduced by the treatment with A paniculata (14.19 0.5 g/L) and ANDLE (15.79 0.4 g/L).

A decreased level of reduced glutahione (GSH) content in liver (2.81 0.1 nmol/mg protein) and bladder (1.20 0.2 nmol/mg protein) after CTX administration was also increased by the treatment with A paniculata (liver: 5.78 0.3 nmol/mg protein; bladder: 2.96 0.2 nmol/mg protein) and ANDLE (liver: 5.14 0.3 nmol/mg protein; bladder: 2.84 0.2 nmol/mg protein).

Production of the proinflammatory cytokine, tumor necrosis factor-, which was elevated during CTX administration, was found to be inhibited by A paniculata and ANDLE treatment.

The lowered level of interleukin-2 and interferon- during CTX treatment was elevated by the administration of A paniculata and ANDLE.

Integrative Cancer Therapies, Vol. 5, No. 3, 244-251 (2006) DOI: 10.1177/1534735406291984 Posted 8/28/06

Chemical Info on Andrographis

Source: Posted 8/08

Increased Tumor Necrosis Factor Alpha & Natural Killer cells

Immunol Invest 5/02

Antiangiogenic: Andrographis paniculata extract & andrographolide

International Immunopharmacology, 2/07

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