Suppression of Proliferation and
Invasive Behavior of Human Metastatic
Breast Cancer Cells by Dietary
Jiahua Jiang, Rachael Wojnowski, Andrej Jedinak and Daniel Sliva
Aim: The study was to evaluate the effect of the dietary supplement BreastDefend (BD) on the proliferation and invasive
behavior of highly metastatic human breast cancer cells in vitro.
Methods: Cell proliferation and cytotoxicity of BD was
evaluated in MDA-MB-231 cells treated with BD (0-40 μg/mL) by MTT assay and trypan blue staining, respectively.
of cell cycle regulatory genes were determined by DNA-microarray analysis. Effect of BD on invasiveness was assessed by
cellular adhesion, migration, and invasion assays. Results: BD treatment of cells MDA-MB-231 resulted in the cytostatic
inhibition of cell proliferation with IC50 22.2, 19.1, and 17.5 μg/mL for 24, 48, and 72 hours, respectively. The inhibition of
proliferation was mediated by the upregulation expression of CCNG1, CHEK1, CDKN1C, GADD45A, and E2F2, whereas BD
downregulated expression of CCNA1 and CDK6 genes.
The induction of expression of GADD45A and inhibition of
expression of cyclin A1 (gene CCNA1) by BD was also confirmed on the protein level. BD treatment suppressed the invasive
behavior of MDA-MB-231 cells by the inhibition of cellular adhesion, migration, and invasion.
This inhibition of invasiveness
was mediated by the suppression of secretion of urokinase plasminogen activator (uPA), and by the downregulation of
expression of CXCR4 in breast cancer cells treated with BD. Conclusion: BD inhibits proliferation and invasive behavior of
the highly metastatic human breast cancer cells in vitro.
BD may have a therapeutic potential for prevention or treatment
of highly metastatic breast cancers.
Integrative Cancer Therapies
9, October 2010
Reprints and permission: http://www.
Ann's NOTE: Very few substances work to kill metastatic cancer cells. Dr. Eliaz who is the creator of this formula also has Modified Citrus Pectin (information on this site -find with SEARCH)that has been shown to kill metastatic prostate cells and in some small studies, to work in men with advanced prostate cancer.
Let's hope funding continues and researchers look at human trials SOON.
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