Carbon Dxde & Human OvaCa Cell Growth

Effect of carbon dioxide on human ovarian carcinoma cell growth

Objective: Laparoscopy may be associated with increased risk of ovarian carcinoma wound metastases. This study was designed to determine whether carbon dioxide exposure increases the growth of human ovarian cancer cells in vitro.

Study Design: Immortalized ovarian epithelial carcinoma cell (SKOV-3 cell line) cultures were exposed to carbon dioxide, nitrous oxide, or culture media with decreased pH for up to 3 hours. Cell growth was determined with the use of a spectrophotometric assay, and the results were compared with control cells by paired t tests and linear regressions analysis.

Results: Carbon dioxide exposure increased SKOV-3 cell growth by 52% after 4 days in culture. The increased cell growth had a linear relationship to the length of carbon dioxide exposure. Cells that were exposed to either nitrous oxide or media with pH 6.3 showed a trend toward decreased growth.

Conclusion: Carbon dioxide exposure increases the in vitro growth of human ovarian carcinoma cells by an effect that is independent of the carbon dioxide–related decrease in the culture media pH.

Am J Obstet Gynecol 2001;185:1314-7.

From the Department of Obstetrics and Gynecology, Indiana University School of Medicine. Presented at the Twenty-seventh Annual Meeting of the Society of Gynecologic Surgeons, Orlando, Fla, March 5-7, 2001.

Reprint requests: William W. Hurd, MD, 128 Apple St, Suite 3800 CHE, Dayton, OH 45409-2793. E-mail: .

Copyright © 2001 by Mosby, Inc.

American Journal of Obstetrics and Gynecology December 2001, part 1 • Volume 185 • Number 6

Transactions Of The Twenty-Seventh Scientific Meeting Of The Society Of Gynecologic Surgeons

Ann's NOTE: This study may indicate support or correlation for the aerobic/anerobic view of cancer cells. That is that cancer cells cannot flourish with oxygen (Otto Warburg won a Nobel Prize for this thesis).

Inhibition of Ca Cells by Artemisinin Derivatives

Pharmacol Res, 9/03

Angiostatic Therapy & Artemisinin
Scutellaria barbatae & Ovarian Ca cells

Gynecol Oncol, 11/03

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